Regenerated buds from in vitro cultured leaves of Peppermint (Mentha x
piperita) have been obtained on a MSM medium supplemented with saccha
rose (20 g.l(-1)) and various concentrations (0.5 mg.l(-1) et 2 mg.l(-
1)) of BAP. Best results were observed using young leaves from in vitr
o micropropagated plants, cultivated in the dark. Different stages of
differentiation during caulogenesis were characterized by Scanning Ele
ctron Microscopy (SEM). After 5 days, a meristematic area developed fr
om the base of the leaf. Ten days further neoformed buds could be obse
rved and a secretory system was early differentiated. Gas Chromatograp
hy (GC) analysis revealed terpene accumulation in the glandular struct
ures.