NMR STRUCTURE AND MUTAGENESIS OF THE FADD (MORT1) DEATH-EFFECTOR DOMAIN

When activated, membrane-bound receptors for Fas and tumour-necrosis f actor initiate programmed cell death by recruiting the death domain of the adaptor protein FADD(1) (Mort1; ref, 2) to the membrane. FADD the n activates caspase 8 (ref. 3) (also known as FLICE4 or MACH(5)) throu gh an interaction between the death-effector domains of FADD and caspa se 8. This ultimately leads to the apoptotic response. Death-effector domains and homologous protein modules known as caspase-recruitment do mains(6) have been found in several proteins(1-14) and are important r egulators of caspase (FLICE) activity and of apoptosis. Here we descri be the solution structure of a soluble, biologically active mutant of the FADD death-effector domain. The structure consists of six antipara llel, amphipathic alpha-helices and resembles the overall fold of the death domains of Fas(15) and p75 (ref. 16). Despite this structural si milarity, mutations that inhibit protein-protein interactions involvin g the Fas death domain have no effect when introduced into the FADD de ath-effector domain. Instead, a hydrophobic region of the FADD death-e ffector domain that is not present in the death domains is vital for b inding to FLICE and for apoptotic activity.