EARLY CHICK-EMBRYOS IN-VITRO

In 1955, Denis New described a technique for the in vitro culture of e arly avian embryos that has formed the basis for nearly all of the exp erimental embryological studies performed on these species since that day. Many modifications to this technique have also been described in these four decades for specific experimental purposes. Here, we review the effects of some parameters that appear to be important for differ ent aspects of the growth of embryos in this type of culture, and cond uct a small experimental comparison between different modifications of the technique as described by various authors. We conclude that the o riginal technique still compares favorably with its alternatives.