SOME ASPECTS OF CHROMATOGRAPHIC BEHAVIOR IN CAPILLARY ELECTROCHROMATOGRAPHY

Retention behavior and column efficiency in capillary electrochromatog raphy (CEC) were compared with those in micro-high-performance liquid chromatography (CL-HPLC). Using a unified microcolumn separation appar atus, capacity factors of 27 neutral solutes in pressurized electrochr omatography (PEC), CEC and mu-HPLC were investigated and no significan t differences were found among these three modes. By linear solvation energy relationship analysis, the same linear equations were obtained in CEC, PEC and mu-HPLC. Systematic investigation of the retention beh aviors under mobile phases with four different kinds of organic solven ts showed that some of the retention rules in HPLC can be applied in C EC for neutral solutes. Gradient elution of ketones and aldehydes is d iscussed. (C) 1998 Elsevier Science B.V.