K. Kawamura, CAPILLARY ELECTROPHORETIC SEPARATION OF MONO-NUCLEOTIDE AND DINUCLEOTIDE OF ADENOSINE USING CYCLODEXTRIN SOLUTIONS WITH MGCL2 ADDITIVE, Journal of chromatography, 802(1), 1998, pp. 167-177
Citations number
31
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
The capillary electrophoretic separation of adenine, adenosine and nuc
leotide isomers of adenosine has been studied using several kinds of b
uffer solutions with cyclodextrin and metal additives in the presence
and absence of sodium dodecyl sulfate. Optimum resolution of 2',5'- an
d 3',5'-adenylyladenosine monophosphate was obtained using beta-cyclod
extrin solution containing 10(-4) M MgCl2. The mixture of adenine, ade
nosine and nine types of adenosine nucleotide was able to be separated
simultaneously within 15 min under the optimum conditions. The method
was applicable to the analysis of the simulation reaction for the pre
biotic formation of adenosine nucleotides. (C) 1998 Elsevier Science B
.V.