COMPARISON OF (-CATECHIN DETERMINATION IN HUMAN PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH 2 TYPES OF DETECTION - FLUORESCENCE AND ULTRAVIOLET())

In this study we developed a high-performance liquid chromatography (H PLC) method for the determination of (+)-catechin in human plasma, usi ng both fluorescence and UV detection. Sample preparation involved pre cipitation of plasma. proteins using acetonitrile, followed by direct injection into the HPLC system using both types of detection. Validati on of accuracy and precision were satisfactory fur both within- and be tween-batch assays. For fluorescence detection, coefficients of variat ion were less than 6.47% and mean relative errors were within +/- 4.8% . The average recovery was 85.31%. The limit of detection and quantifi cation were 5 ng/ml and 40 ng/ml, respectively. Ultraviolet detection was also used bur appeared less sensitive and selective than fluoresce nce detection. This new method provides a simple, accurate. precise an d specific method for the determination for (+)-catechin in plasma. (C ) 1998 Elsevier Science B.V.