Citation
Ecy. Chan et Pc. Ho, ENANTIOMERIC SEPARATION OF TRAMADOL HYDROCHLORIDE AND ITS METABOLITESBY CYCLODEXTRIN-MEDIATED CAPILLARY-ZONE-ELECTROPHORESIS, Journal of chromatography B. Biomedical sciences and applications, 707(1-2), 1998, pp. 287-294
Citations number
20
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Journal of chromatography B. Biomedical sciences and applications
ISSN journal
13872273
→ ACNP
Volume
707
Issue
1-2
Year of publication
1998
Pages
287 - 294
Database
ISI
SICI code
0378-4347(1998)707:1-2<287:ESOTHA>2.0.ZU;2-H
Abstract
Thr enantiomeric separation of tramadol hydrochloride and its major me
tabolites, O-demethyltramadol (M1) and N-demethyltramadol (M2) was: st
udied using cyclodextrin (CD)-mediated capillary zone electrophoresis
(CZE). Influence of the choice of type and concentration of CD, capill
ary temperature, length of capillaries, buffer pH and the addition of
polymer modifier on the chiral separation of tramadol and its metaboli
tes was evaluated. It was found that the drug and the metabolites can
be baseline-separated simultaneously by using 50 mM phosphate buffer (
pH 2.5) containing 75 mM methyl-beta-CD, 220 mM urea and 0.05% (w/v) h
ydroxypropylmethyl cellulose. (C) 1998 Elsevier Science B.V.