Ad. Cox et al., STRUCTURAL-ANALYSIS OF THE PHASE-VARIABLE LIPOOLIGOSACCHARIDE FROM HAEMOPHILUS-SOMNUS STRAIN-738, European journal of biochemistry, 253(2), 1998, pp. 507-516
The structure of the phase variable lipooligosaccharide (LOS) from Hae
mophilus somnus strain 738 was elucidated. The LOS was subjected to a
variety of degradative procedures. The structures of the purified prod
ucts were established by monosaccharide and methylation analyses, NMR
spectroscopy and mass spectrometry. The following structures for the t
wo major components were determined on the basis of the combined data
from these experiments. [GRAPHICS] In the structures Kdo is 3-deoxy-D-
manno-octulosonic acid, PEtn is phosphoethanolamine, PCho is phosphoch
oline, Hep is L-glycero-D-manno-heptose, and the remaining glucose uni
ts have the D configuration. The elucidation of these structures has i
ncreased our understanding of the relationship between the phase-varia
ble LOS and the pathogenic potential of this organism.