Archaea were, until recently, considered to be confined to specialized
environments including those at high temperature, high salinity, extr
emes of pH and ambients that permit methanogenesis. Recently developed
molecular methods for studying microbial ecology, which do not necess
itate cell culturing, have demonstrated their presence in a wide varie
ty of temperate and cold environments including agricultural and fores
t soils, fresh water lake sediments, marine picoplankton and deep-sea
locations. These discoveries mark the beginning of a new era for inves
tigating the Archaea and in particular their physiological and metabol
ic properties and their biological roles in complex microbial populati
ons.