IN-VIVO P-31 NMR OSIRIS OF BIOENERGETIC CHANGES IN RABBIT KIDNEYS DURING AND AFTER ISCHEMIA - EFFECT OF PRETREATMENT WITH AN INDENO-INDOLE COMPOUND

Changes in energy phosphates of rabbit kidneys subjected to ischaemia- reperfusion have been measured in vivo with volume selective P-31 NMR spectroscopy. The effects of pretreatment with a new lipid peroxidatio n inhibitor (indeno-indol derivate - code name H290/51) on the bioener getic changes were analysed. The left kidney was moved to a subcutaneo us pocket to facilitate exact positioning over the surface coil. A H-1 NMR image was acquired and a 3.5-mL cube selected for P-31 NMR spectr a. P-31 NMR spectra were recorded before occlusion of the left renal a rtery, during 1 h of ischaemia and 2 hours of reperfusion. Ischaemia i nduced drastic changes in the levels of inorganic phosphates and ATP a s well as intracellular acidosis. A normalization was observed during reperfusion. Two hours after reperfusion significantly higher values f or beta-ATP/Pi and intracellular pH were recorded in the animals pretr eated with H290/51. The present technique allows quantitative analyses of changes in kidney bioenergetics in vivo during different experimen tal conditions. The importance of ischaemia-reperfusion induced lipid peroxidation for mitochondrial function is emphasized.