KINETICS OF IL-8, MIP-1-ALPHA, TNF-ALPHA, IL-1-BETA, IL-1RA AND IL-10IN HUMAN WHOLE-BLOOD SAMPLES TRIGGERED BY SMOOTH AND ROUGH LPS

An in vitro model for the study of sepsis mediators was used to invest igate the effects of two different lipopolysaccharides (LPS), a smooth (LPS-S) and a rough (LPS-R) type, on the release of chemokines (IL-8 and MLP-1 alpha) and cytokines (TNF alpha, IL-1 beta, IL-1ra and IL-10 ) from human whole blood samples. TNF alpha level increased significan tly vs, control, at 4 h and 8 h after the challenge with smooth and ro ugh type of LPS respectively. Concentrations of the two chemokines stu died, IL-8 and MIP-la, were significantly elevated following stimulati on by both LPS, and reached concentrations significantly different fro m controls at 4, 8 and 24 h. After 24 h of incubation both LPS produce d a significant IL-10 increase, although such change was more substant ial with the rough type. Present data suggest an early and maintained release of chemokines regardless of the type of LPS used and often in absence of a significant increase in primary pro-inflammatory cytokine s.