Fr. Lauritsen et A. Lunding, A STUDY OF THE BIOCONVERSION POTENTIAL OF THE FUNGUS BJERKANDERA-ADUSTA WITH RESPECT TO A PRODUCTION OF CHLORINATED AROMATIC-COMPOUNDS, Enzyme and microbial technology, 22(6), 1998, pp. 459-465
Fluoro-labelled substrates and membrane inlet mass spectrometry (MIMS)
was used to test the bioconversion potential of the white-rot fungus
Bjerkandera adusta with respect to a production of novel halogenated a
romatic compounds. The presence of a fluorine atom on the aromatic rin
g of possible precursors to the well-known metabolites, 3-chloro-4-met
hoxybenzaldehyde and 3,5-dichloro-4-methoxybenzaldehyde, did not influ
ence the metabolism and they all gave mixed chloro-fluoro-methoxybenza
ldehydes; however, we were unable to produce any new chlorinated compo
unds. Neither supplementation of anisole, benzaldehyde, veratryl alcoh
ol, the naphthalene analogs nor methyl-substituted benzaldehydes resul
ted in a production of detectable amounts of the corresponding chlorin
ated products. Apparently, only hydroxybenzaldehydes and/or methoxyben
zaldehydes are chlorinated by the halogenating system. Experiments whe
re an aromatic alcohol, aldehyde, or the analog acid was supplemented
gave qualitatively and quantitatively similar membrane inlet mass spec
tra suggesting that oxidases and reductases maintain a stoichiometric
ration between these compounds. Several cases of hydroxylation and/or
methoxylation of the substrates were also observed. (C) 1998 Elsevier
Science Inc.