T. Mennini et al., SPINAL-CORD GLT-1 GLUTAMATE TRANSPORTER AND BLOOD GLUTAMIC-ACID ALTERATIONS IN MOTOR-NEURON DEGENERATION (MND) MICE, Journal of the neurological sciences, 157(1), 1998, pp. 31-36
This study characterizes for the first time neurochemical mechanisms i
n Mnd mice, initially described as a model of motor neuron disease and
more recently proposed as a model for neuronal ceroid lipofuscinosis.
A selective decrease (-30%) of [H-3]glutamate uptake was found in spi
nal cord but not cortical synaptosomes of Mnd mice aged 28 weeks, when
they show histopathological alterations, complete blindness and moder
ate neurological deficits. In spite of the widespread presence of stor
ed material in neurons in many brain regions and spinal cord, the acti
ve transport of [H-3]serotonin, [H-3]dopamine and depolarization-induc
ed [H-3]serotonin release were not affected. Spinal EAACl glutamate tr
ansporter protein was significantly decreased in some but not all aged
mice by 36% on average, possibly due to the loss of motor neurons. GL
T-1 immunoreactivity was reduced by 34% in 28-week-old Mnd mice, while
GLAST immunoreactivity was not affected. In Mnd mice aged 14 weeks, w
hen there was no apparent alteration of motor function, the defect in
the glial transporter protein GLT-1 was similar to that in 28-week-old
mice (25%). Blood glutamic acid concentration was increased in Mnd mi
ce aged 14-22 weeks. We suggest that the early decrease of GLT-1 prote
in might raise the extrasynaptic glutamic acid concentration, and cont
ribute to the loss of motor neurons in affected mice, resulting in low
[H-3]glutamate uptake, low EAACl immunoreactivity and neurological de
ficits. (C) 1998 Elsevier Science B.V.