AUTOLYTIC SITE MUTANT R105C OF RAT TRYPSIN

The autolytic site Arg105 of rat trypsin was replaced with Cys by DNA site-directed mutagenesis method. Comparison of expression and purific ation of R105C trypsin along with the wild type and some other Arg105 mutants indicates that R105C trypsin could be expressed as well but wi th a lower expression level. It is unexpected that R105C trypsin has n o detectable activity toward trypsin substrate TAME, quite different f rom the wild type and other Arg1O5 mutants. Native gel electrophoresis analysis indicates that R105C trypsin has a similar mobility rate to that of wild type trypsin. FPLC also gives similar retaining time. The loss of activity of R105C trypsin may result from the conformational change around active site, but not the dimer formation.