NUCLEOTIDE-SEQUENCE OF CDNA FRAGMENTS CODING FOR THE FAD-BINDING, GLYCEROPHOSPHATE-BINDING AND CALCIUM-BINDING DOMAINS OF HUMAN ISLET MITOCHONDRIAL GLYCEROPHOSPHATE DEHYDROGENASE

The cDNA fragments coding for the FAD-, glycerophosphate-and calcium-b inding domains of mitochondrial glycerophosphate dehydrogenase (mGDH) were synthetized using RNA extracted from freshly isolated pancreatic islets of a normal subject and two-non-insulin-dependent diabetic pati ents. Single strand conformation polymorphism analysis of the PCR prod ucts yielded the same mobility as control cDNA probes. Likewise, the n ucleotide sequence and corresponding amino acid sequence were identica l to the normal gene bank sequence. These findings argue against the p resence, in pancreatic islets, of an mGDH isoform distinct from that p reviously characterized in extrapancreatic organs.