IDENTIFICATION, PURIFICATION, AND PARTIAL CHARACTERIZATION OF A FACTOR FROM RABBIT SERUM THAT INHIBITS PROLACTIN SECRETION BY PITUITARY-CELLS CULTURED IN-VITRO

Authors
GIACOMELLI S CHENG CY LEONE MG PALMERY M SILVESTRINI B
Citation
S. Giacomelli et al., IDENTIFICATION, PURIFICATION, AND PARTIAL CHARACTERIZATION OF A FACTOR FROM RABBIT SERUM THAT INHIBITS PROLACTIN SECRETION BY PITUITARY-CELLS CULTURED IN-VITRO, Biochemistry and molecular biology international, 42(6), 1997, pp. 1199-1213
Citations number
19
Journal title
Biochemistry and molecular biology international → ACNP
ISSN journal
10399712
Volume
42
Issue
6
Year of publication
1997
Pages
1199 - 1213
Database
ISI
SICI code
1039-9712(1997)42:6<1199:IPAPCO>2.0.ZU;2-#
Abstract
A biological factor that inhibited prolactin secretion by pituitary ce lls cultured in vitro was identified, purified, and partially characte rized from normal rabbit serum. This biological factor was also found to potentiate dopamine-mediated aortic contraction using rabbit aortic strips in vitro. Following SDS-PAGE, this factor displayed an apparen t Mr of 17 kDa, which is different from the Mr of most known endogenou s factors having an inhibiting activity on pituitary prolactin secreti on, suggesting that this may be a yet-to-be identified novel molecule.