ESTABLISHMENT OF A HIGHLY EFFICIENT REGENERATION SYSTEM FROM LEAF BASE SEGMENTS OF OAT (AVENA-SATIVA L.)

A reliable and efficient protocol for the regeneration of fertile plan ts derived from leaf base segments of young in-vitro-grown oat seedlin gs has been developed successfully. Callus induction and shoot regener ation were achieved when the basal region of young seedlings was cultu red on auxin-containing medium. Callus induction efficiencies as well as regeneration frequencies were correlated with the developmental sta ge and the genotype nf thp explants. In five different genotypes nf oa t, an average of 25 plants per explant could be produced and for the m ost responsive genotype more than 50 regenerants per explant could be regenerated reproducibly. This high regeneration potential makes oat l eaf bases a very attractive target for transformation.