HIGH FORSKOLIN PRODUCTION IN HAIRY ROOTS OF COLEUS-FORSKOHLII

Hairy roots of Coleus forskohlii were induced by infection with the Ag robacterium rhizogenes MAFF 03-01724 strain. Growth and forskolin prod uction of two hairy root clones cultured in various liquid media were examined. Hairy root clone B9 grew well in woody plant liquid medium a nd showed a high forskolin yield (ca. 1.3 mg/100 mi flask) after 5 wee ks of culture. The time course of growth and forskolin production of t he clone B9 cultured in woody plant liquid medium was also examined. R apid growth started at week 2 and continued until week 5. The highest forskolin yield (ca. 1.6 mg/100 mi flask) was obtained at week 5. Prod uctivity was much higher than that previously reported.