OCT-1 PROMOTER REGION CONTAINS OCTAMER SITES AND TAAT MOTIFS RECOGNIZED BY OCT PROTEINS

The 5'-upstream region (1.3 kh) of the gene encoding the POU domain tr anscription factor Oct-1 was cloned and sequenced, CAT reporter gene a nalysis of this region has detected a functionally active promoter. Th is region contains 24 TAAT-core sites, arranged in five clusters (four to sis sites in one cluster); two octamer sites (ATGCAAAT) are locate d in the first and second clusters; in the second one the CCAAT-box ad jacent to the octamer overlaps with the TAAT-core site. As shown by ge l retardation assay, Oct-1, Oct-2, and some unknown proteins from myel oma cell line NS/0 interact with the TAAT-core sites of these clusters , The results suggest autoregulation of Oct-1 gene expression that may also be controlled by other POU proteins, homeodomain proteins and CC AAT trans-action factors, (C) 1998 Federation of European Biochemical Societies.