ANTIGENIC COMPONENTS OF SOMATIC EXTRACT FROM ADULT FASCIOLA-GIGANTICARECOGNIZED BY INFECTED HUMAN SERA

The antigenic components of Fasciola gigantica somatic extract were re vealed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis ( SDS-PAGE) and immunoblotting technique using sera from patients with F . gigantica infection, patients with clinically diagnosed fascioliasis , patients with other infections/illness and healthy adults. By SDS-PA GE, it was found that the somatic product comprised more than 22 polyp eptides, Immunoblotting analysis revealed at least 13 components which were strongly recognized by sera of patients with fascioliasis. These antigenic components had molecular weights ranging from less than 14. 4 to more than 94 kDa, One antigenic component, i.e. 38 kDa was found to give a consistent reaction with sera of patients with fascioliasis (100% sensitivity and 96.7% specificity). The finding suggests that th e 38 kDa components may be a potential diagnostic antigen for fascioli asis.