H. Kallstrom et Ab. Jonsson, CHARACTERIZATION OF THE REGION DOWNSTREAM OF THE PILUS BIOGENESIS GENE PILC1 IN NEISSERIA-GONORRHOEAE, Biochimica et biophysica acta, N. Gene structure and expression, 1397(2), 1998, pp. 137-140
The nucleotide sequence of a 3 kb region downstream of pilC1 in Neisse
ria gonorrhoeae MS11 was analyzed. This region contains two open readi
ng frames, ORF1 and ORF2, and several repetitive DNA elements. ORF1 en
codes an outer membrane protein that shows homology to orf98 of Pedioc
occus acidilactici. PCR with primers specific for ORF1 revealed that t
he gene is present in all gonococcal strains tested. The other open re
ading frame, ORF2, is highly homologous to the putative integral membr
ane protein HI1680 of Haemophilus influenzae. (C) 1998 Elsevier Scienc
e B.V.