ANALYSIS AND OVEREXPRESSION IN ESCHERICHIA-COLI OF A STAPHYLOCOCCAL GENE ENCODING SERYL-TRANSFER-RNA SYNTHETASE

Authors
BAUSCH N SEIGNOVERT L BEAULANDE M LEBERMAN R HARTLEIN M
Citation
N. Bausch et al., ANALYSIS AND OVEREXPRESSION IN ESCHERICHIA-COLI OF A STAPHYLOCOCCAL GENE ENCODING SERYL-TRANSFER-RNA SYNTHETASE, Biochimica et biophysica acta, N. Gene structure and expression, 1397(2), 1998, pp. 169-174
Citations number
16
Categorie Soggetti
Biology,Biophysics
Journal title
Biochimica et biophysica acta, N. Gene structure and expression → ACNP
ISSN journal
01674781
Volume
1397
Issue
2
Year of publication
1998
Pages
169 - 174
Database
ISI
SICI code
0167-4781(1998)1397:2<169:AAOIEO>2.0.ZU;2-Q
Abstract
We have sequenced and expressed in Escherichia coli the gene encoding the seryl-tRNA synthetase from the pathogenic bacterium Staphylococcus aureus. The overexpressed and purified recombinant enzyme was able to aminoacylate unfractionated tRNA from E. coli. Its activity was not a ffected by antibodies raised against and inhibiting the E. coli seryl- tRNA synthetase. (C) 1998 Elsevier Science B.V.