A NEW DROSOPHILA ULTRAVIOLET LIGHT-DAMAGED DNA RECOGNITION ENDONUCLEASE THAT SELECTIVELY NICKS A (6-4)-PHOTOPRODUCT SITE

Authors
KAI M TODO T WADA M RYO H MASUTANI C KOBAYASHI H MORIOKA H OHTSUKA E HANAOKA F SAKAGUCHI K
Citation
M. Kai et al., A NEW DROSOPHILA ULTRAVIOLET LIGHT-DAMAGED DNA RECOGNITION ENDONUCLEASE THAT SELECTIVELY NICKS A (6-4)-PHOTOPRODUCT SITE, Biochimica et biophysica acta, N. Gene structure and expression, 1397(2), 1998, pp. 180-188
Citations number
34
Categorie Soggetti
Biology,Biophysics
Journal title
Biochimica et biophysica acta, N. Gene structure and expression → ACNP
ISSN journal
01674781
Volume
1397
Issue
2
Year of publication
1998
Pages
180 - 188
Database
ISI
SICI code
0167-4781(1998)1397:2<180:ANDULD>2.0.ZU;2-S
Abstract
We have previously described the purification of an ultraviolet light (UV) damage-specific DNA-binding protein from Drosophila melanogaster, designated D-DDB P1 [Nucleic Acids Res.. 23 (1995) 2600-2607]. Here, we obtained highly purified D-DDB P1 from Drosophila Kc cells, and we found that D-DDB P1 is also a nuclease. D-DDB P1 can selectively bind to pyrimidine (6-4) pyrimidone photoproducts, and in the presence of M g++. D-DDB P1 can catalyze an incision immediately on the 3' and 5' si des of, the (6-4) photoproduct site. (C) 1998 Elsevier Science B.V.