T. Brennan et al., 2-DIMENSIONAL PARALLEL ARRAY TECHNOLOGY AS A NEW APPROACH TO AUTOMATED COMBINATORIAL SOLID-PHASE ORGANIC-SYNTHESIS, Biotechnology and bioengineering, 61(1), 1998, pp. 33-45
An automated, 96-well parallel array synthesizer for solid-phase organ
ic synthesis has been designed and constructed. The instrument employs
a unique reagent array delivery format, in which each reagent utilize
d has a dedicated plumbing system. An inert atmosphere is maintained d
uring all phases of a synthesis, and temperature can be controlled via
a thermal transfer plate which holds the injection molded reaction bl
ock. The reaction plate assembly slides in the X-axis direction, while
eight nozzle blocks holding the reagent lines slide in the Y-axis dir
ection, allowing for the extremely rapid delivery of any of 64 reagent
s to 96 wells. In addition, there are six banks of fixed nozzle blocks
, which deliver the same reagent or solvent to eight wells at once, fo
r a total of 72 possible reagents. The instrument is controlled by sof
tware which allows the straightforward programming of the synthesis of
a larger number of compounds. This is accomplished by supplying a gen
eral synthetic procedure in the form of a command file, which calls up
on certain reagents to be added to specific wells via lookup in a sequ
ence file. The bottle position, flow rate, and concentration of each r
eagent is stored in a separate reagent table file. To demonstrate the
utility of the parallel array synthesizer, a small combinatorial libra
ry of hydroxamic acids was prepared in high throughput mode for biolog
ical screening. Approximately 1300 compounds were prepared on a 10 mu
mole scale (3-5 mg) in a few weeks. The resulting crude compounds were
generally >80% pure, and were utilized directly for high throughput s
creening in antibacterial assays. Several active wells were found, and
the activity was verified by solution-phase synthesis of analytically
pure material, indicating that the system described herein is an effi
cient means for the parallel synthesis of compounds for lead discovery
. (C) 1998 John Wiley & Sons, Inc.