TECHNICAL NOTE - IMPROVED DNA EXTRACTION FROM ANCIENT BONES USING SILICA-BASED SPIN COLUMNS

Authors
YANG DY ENG B WAYE JS DUDAR JC SAUNDERS SR
Citation
Dy. Yang et al., TECHNICAL NOTE - IMPROVED DNA EXTRACTION FROM ANCIENT BONES USING SILICA-BASED SPIN COLUMNS, American journal of physical anthropology, 105(4), 1998, pp. 539-543
Citations number
8
Categorie Soggetti
Anthropology,"Art & Humanities General",Mathematics,"Biology Miscellaneous
Journal title
American journal of physical anthropology → ACNP
ISSN journal
00029483
Volume
105
Issue
4
Year of publication
1998
Pages
539 - 543
Database
ISI
SICI code
0002-9483(1998)105:4<539:TN-IDE>2.0.ZU;2-0
Abstract
We describe a simple method for extracting polymerase chain reaction-a mplifiable DNA from ancient bones without the use of organic solvents. Bone powders are digested with proteinase K, and the DNA is purified directly using silica-based spin columns (QIAquick(TM), QIAGEN). The e fficiency of this protocol is demonstrated using human bone samples ra nging in age from 15 to 5,000 years old. (C) 1998 Wiley-Liss, Inc.