EXPRESSION OF NERVE GROWTH-FACTOR AND NEUROTROPHIN-3 MESSENGER-RNAS IN HIPPOCAMPAL INTERNEURONS - MORPHOLOGICAL CHARACTERIZATION, LEVELS OFEXPRESSION, AND COLOCALIZATION OF NERVE GROWTH-FACTOR AND NEUROTROPHIN-3

Citation
M. Pascual et al., EXPRESSION OF NERVE GROWTH-FACTOR AND NEUROTROPHIN-3 MESSENGER-RNAS IN HIPPOCAMPAL INTERNEURONS - MORPHOLOGICAL CHARACTERIZATION, LEVELS OFEXPRESSION, AND COLOCALIZATION OF NERVE GROWTH-FACTOR AND NEUROTROPHIN-3, Journal of comparative neurology, 395(1), 1998, pp. 73-90
Citations number
64
Categorie Soggetti
Neurosciences,Zoology
ISSN journal
00219967
Volume
395
Issue
1
Year of publication
1998
Pages
73 - 90
Database
ISI
SICI code
0021-9967(1998)395:1<73:EONGAN>2.0.ZU;2-8
Abstract
We have investigated the distribution and morphology of hippocampal in terneurons that express the neurotrophins nerve growth factor (NGF) an d neurotrophin-3 (NT-3) in the rat. For this study, we combined in sit u hybridization for the detection of NGF and NT-3 mRNAs and immunocyto chemistry against the calcium-binding proteins parvalbumin (PARV), cal retinin (CALR), and calbindin (CALB). Whereas the majority of PARV(+) interneurons expressed NGF mRNA, only subsets of CALR- and CALB-immuno reactive interneurons (23% and 24%, respectively) displayed NGF hybrid ization. Most CALB/NGF(+) cells were located in the stratum oriens/alv eus of the CA3-CA1 regions, suggesting that they may include the popul ation of CALB(+), hippocamposeptal, nonpyramidal neurons. Most of the nonspiny CALR/NGF(+) neurons were located within or in the vicinity of the pyramidal layer and had faint CALR immunostaining and stellate, t hin dendrites. Regarding the spiny CALR-immunoreactive cells, we found that most of these neurons in the hilus were NGF(+), whereas only 59% of displayed NGF hybridization in the stratum lucidum of the CA3 regi on. A small subset of PARV- and CALR-immunoreactive cells expressed NT -3 mRNA (16% and 13%, respectively). NT-3 message was not found in the large basket cells of the dentate gyrus, whereas the distribution and morphology of CALR/NT-3(+) cells were similar to those of nonspiny CA LR/NGF(+) cells. In fact, double in situ hybridization analysis confir med that most NT-3(+) neurons also expressed NGF mRNA, indicating coex pression of both neurotrophins in subpopulations of PARV(+) and CALR() neurons. Moreover, the level of NGF mRNA expression was higher in PA RV(+) neurons than in CALR- and CALB-immunoreactive interneurons, wher eas NT-3 message was expressed similarly in PARV(+) and CALR(+) neuron s. The present findings show a differential expression of NGF and NT-3 mRNAs in subsets of hippocampal interneurons and suggest that the exp ression of these transcripts depends on factors intrinsic to particula r cell types. (C) 1998 Wiley-Liss, Inc.