Sj. Oh et al., SEQUENCING AND CHROMOSOMAL LOCALIZATION OF THE RA138 GENE ENCODING A RICE ALLERGENIC PROTEIN, Molecules and cells, 8(2), 1998, pp. 175-180
A cDNA clone (RA138) encoding a rice allergenic (RA) protein has been
isolated during a large-scale random sequencing of a cDNA library prep
ared from developing seeds. The nucleotide sequence of the RA138 gene
contained an open reading frame (ORF, 477 bp) encoding a 17 kDa protei
n. The amino acid sequence deduced from the ORF was composed of 159 am
ino acid residues and was highly homologous to those from RA genes pre
viously isolated, such as RA5 (92% identity), RA14 (73%), and RA17 (68
%), The protein contained 10 cysteine residues that were conserved in
the alpha-amylase/trypsin inhibitor family including RA proteins. Excl
uding a putative signal peptide consisting of 26 amino acid residues,
the mature protein would be 14.4 kDa in size and have a pi of 7.0. DNA
gel blot analysis under high stringency conditions indicated that mul
tiple copies of the RA138 gene were present in the rice genome. The ch
romosomal location of the RA138 gene has been identified on chromosome
7 in a segregation analysis using a population of 164 recombinant inb
red lines derived from a cross between Milyang 23 and Gihobyeo, The lo
cus that may contain multiple copies of the RA138 was located between
RFLP markers RG477A and C492 with genetic distances of 10.7 cM and 6.7
cM, respectively.