EFFECT OF ADDING PROPIONIBACTERIUM-SHERMANII NCDO-853 OR LACTOBACILLUS-CASEI SSP. CASEI IFPL-731 ON PROTEOLYSIS AND FLAVOR DEVELOPMENT OF CHEDDAR CHEESE

Proteolysis and flavor development were monitored in Cheddar cheese ma de from milk inoculated with Lactobacillus casei ssp. casei IFPL 731 ( 10(4) cfu/ml cheesemilk) or Propionibacterium shermanii NCDO 853 at th ree different levels (10(5), 10(6), and 10(7) cfu/mL cheesemilk). The pH and chemical composition of the experimental cheeses were not affec ted by the addition of L. casei or Prop. shermanii. Cheeses inoculated with L. casei or Prop. shermanii at low and medium levels received th e best scores for flavor development and body. However, the addition o f Prop. shermanii at a high level caused a sweet and nutty flavor. Ure a-PAGE, WSN and RP-HPLC showed minor differences in proteolysis betwee n the control and experimental cheeses. Major differences were found a t the amino acid level. In general, the content of amino acid increase d as the Propionibacterium inoculum increased in the experimental chee ses. Besides, a decrease in the content of hydrophobic peptides was ob served in cheeses inoculated with Prop. shermanii at medium and high l evels. The increase in amino acid content was not apparent when L. cas ei was added to cheese, possibly due to the lack of cell lysis. There is an optimum inoculum level of Prop. shermanii above which the flavor of Cheddar cheese resembles that of Swiss-type cheese.