Mk. Hammouri et al., QUANTITATIVE-ANALYSIS OF FRUCTOSE FATE IN A PLANT FERMENTATION SYSTEM, Journal of agricultural and food chemistry, 46(4), 1998, pp. 1428-1432
There is a need for studies on the fermented plant hairy root cultures
to learn more about the consumption and production of carbohydrates,
sucrose, glucose, and fructose trends during plant metabolism and grow
th in fermented and biological systems. The multivariant quantitative
analysis of sugars was carried out using a Fourier transform infrared
spectrophotometer combined with a cylindrical internal reflection cell
and a dedicated computer. Another analysis of the same samples was pe
rformed according to an enzymatic method. The obtained results were co
mpared with the data of the spectrophotometry with very good agreement
. Results achieved provide a new insight into the metabolism of cell c
ultures. The results demonstrate the possibility of analysis of fructo
se concentration in mixtures of glucose, sucrose, and various constitu
ents of the media. Sucrose concentrations of 88 or 176 mM were diminis
hed after 12 and 24 days, respectively, whereas glucose increased init
ially to reach maxima and fructose was building up steadily throughout
the duration of study (28 days). The hairy roots were unable to consu
me fructose as assumed previously.