HOMOLOG OF A-FACTOR RECEPTOR GENE IN SACCHAROMYCES-EXIGUUS

Homologs of Saccharomyces cerevisiae STE3, a-factor receptor gene were detected from S. exiguns NFRI 3539 by low stringency Southern hybridi zation. This strain might have at least two types of homolog. One of t hese homologs, designated as e-STE3 was cloned. Its nucleotide sequenc e revealed 60% identity to STE3. The putative protein coding region co nsisted of 453 amino acid residues. The amino acid sequence identity b etween STE3 and e-STE3 was 62%, and that of the N-terminal 303 amino a cid residues considered to be the pheromone binding domain was 79%. Th e e-STE3 sequence submitted to the DDBJ/EMBL/GenBank data libraries is available under Accession Number AB003086, (C) 1998 John Wiley & Sons , Ltd.