PREVIOUS studies in adult rats have demostrated that the neurotrophin,
brain-derived neurotrophic factor (BDNF), is present in virtually all
cells of the pituitary intermediate lobe. In the present study, we de
monstrate that cells cultured from adult intermediate lobe pituitary (
ILP) rapidly lose their BDNF immunoreactivity (IR). Furthermore, a sim
ilar loss of immunostaining occurs in whole (undissociated) ILP within
30 min after removal from the rat. However, when the dopamine agonist
apomorphine is present throughout the dissociation procedure and duri
ng cultivation, BDNF-IR is preserved. Supplying apomorphine only durin
g either dissociation or cultivation did not prevent the loss of BDNF-
IR in the 24 h cultures. These results suggest that a tonic dopaminerg
ic stimulus is required to maintain BDNF-IR in ILP cells.