EXPERIMENTAL AND THEORETICAL-STUDIES ON G RADIENT CHROMATOGRAPHY OF PROTEINS

Preparative liquid column chromatography is a powerful method for the isolation of proteins from complex mixtures. The use of solvent gradie nts has proved invaluable in the optimisation of the chromatographic s eparation process. In contrast to isocratic chromatography, the elutin g strength of the solvent is increased during the separation. To this end, an increasing proportion of a solvent of high eluting strength is added to solvent of low eluting strength. This publication is intende d to contribute to the general understanding of and to a quantitative description of preparative gradient chromatography of dissolved protei ns. The analysis is based on the experimental determination of various chromatograms of the model protein lysozyme on a cation exchanger.