Wh. Goldmann et Z. Guttenberg, EXAMINATION OF TEMPERATURE-INDUCED GEL-SOL TRANSFORMATION OF ALPHA-ACTININ CROSS-LINKED ACTIN NETWORKS BY STATIC LIGHT-SCATTERING/, FEBS letters, 426(2), 1998, pp. 255-259
We studied the gel-sol transformation of F-actin/alpha-actinin solutio
ns. Gross-linking of actin filaments by a-actinin shows a temperature-
dependent increase in light scatter signal, (I)T. Higher F-actin/alpha
-actinin molar ratios, r(A alpha) as well as increases in F-actin conc
entration, [A], and reduction of actin filament lengths, r(AG), augmen
t the maximal light intensity, I and shift the gel-sol transition poin
t, T-g to higher temperatures. This behavior is interpreted in terms o
f the model developed by Tempel, M., Isenberg, G. and Sackmann, E. (19
96) (Physical Review E 54, 1802-1810) based on the percolation theory.
Using the temperature-dependent binding model of this theory allows i
nstant prediction of the equilibrium constant, K for F-actin/alpha-act
inin solutions at temperatures T< T-g. (C) 1998 Federation of European
Biochemical Societies.