PREPARATION OF ORGANIZED PROTEIN MULTILAYERS

A novel technique of preparation of multilayer assemblies consisting o f a controlled number of molecular layers of various proteins arranged according to a predetermined architecture is described. The multilaye rs were formed by consecutive adsorption of polyanions and positively charged proteins below their isoelectric point and covalently crosslin ked via protein amino groups. Polyanions were washed out from the cros slinked protein assembly by rinsing with buffer in which the net charg e of the proteins was inverted.