IMPROVED PERFORMANCE OF AMBERLITE IRA-904 IMMOBILIZED GLUCOAMYLASE

When the glucoamylase protein immobilized to Amberlite IRA-904 was est imated by direct (Kjeldhal), indirect (measuring the protein left in t he supernatant) and elution methods, direct and indirect methods gave almost same results while the elution method gave a protein concentrat ion of only 68.9% of that obtained with the Kjeldhal method. When the activity of the soluble and immobilized glucoamylase were compared wit h different substrates, the activity yield increased with decreasing m olecular size of the substrate. However when the immobilized glucoamyl ase containing Amberlite IRA-904 was pound, it Showed increase in acti vity with increasing molecular weight of the substrates.