IN-VITRO SYNTHESIS OF OLIGOSACCHARIDES BY ACCEPTOR REACTION OF DEXTRANSUCRASE FROM LEUCONOSTOC-MESENTEROIDES

Glucose was used as acceptor to obtain small chain oligossaccharides f rom sucrose using dextransucrase from Leuconostoc mesenteroides NRRL B -512F. Better conditions for the synthesis of the oligosaccharides wer e obtained using experimental design and response surface methodology. Yield of oligosaccharides was increased from 5% to 45% following an i ncrease in both sucrose and glucose/sucrose concentrations, from 58 g/ l to 142 g/l and from 0.02 to 0.18, respectively. Molecular weight inc reased from 2800 to 4500 daltons with a temperature shifting form 10 d egrees C to 30 degrees C.