PUTATIVE LOCATION OF COMMON REGION AND COAT PROTEIN GENE OF BLACKGRAMISOLATE OF MUNGBEAN YELLOW MOSAIC GEMINIVIRUS

Reciprocal cross hybridization between DNA-A and DNA-B molecules showe d that MYMV genome contains certain regions common to each other. ''Co mmon region'' of MYMV belongs to the 0.55 kb Kpnl-Clal fragment of DNA -A and 0.25 kb Xbal-Hind III fragment of DNA-B. Hybridization of DNA-A restriction fragments with the full length coat protein region probe to Indian cassava mosaic virus (ICMV) showed the location of MYMV coat protein coding region in DNA-A. The greater extent of homology of ICM V coat protein region tails within the 400 base pair region of Clal-Cl al fragment in DNA-A of MYMV-Bg. The homology between ICMV and MYMV-Bg coat protein gene appeared to be less than 50%. Cross hybridization b etween DNA-A and DNA-B molecules helped to trim out the homologous reg ion and the DNA-A and DNA-B specific probes could be tailored.