Is. Sheoran et Hs. Saini, A SIMPLE METHOD FOR IN-VIVO PROTEIN LABELING IN INTACT RICE PLANT ANTHERS - WATER-STRESS EFFECTS ON PROTEIN-SYNTHESIS, Journal of Plant Biochemistry and Biotechnology, 7(1), 1998, pp. 47-50
Three methods for in vivo protein labelling of rice anthers were teste
d. The 'wick' method was found to incorporate 5- and 9-fold higher amo
unt of labelled S-35-methionine into proteins as compared to the other
two methods. This method was quite effective, safe and easy to use wi
th any kind of tissue or plant species with reproducible results. Two
dimensional polyacrylamide gel electrophoresis the qualitative changes
in rice anther protein synthesis under water stress was used to test
the reproducibility of the method.