A SIMPLE METHOD FOR IN-VIVO PROTEIN LABELING IN INTACT RICE PLANT ANTHERS - WATER-STRESS EFFECTS ON PROTEIN-SYNTHESIS

Citation
Is. Sheoran et Hs. Saini, A SIMPLE METHOD FOR IN-VIVO PROTEIN LABELING IN INTACT RICE PLANT ANTHERS - WATER-STRESS EFFECTS ON PROTEIN-SYNTHESIS, Journal of Plant Biochemistry and Biotechnology, 7(1), 1998, pp. 47-50
Citations number
12
Categorie Soggetti
Biology,"Biothechnology & Applied Migrobiology","Plant Sciences
ISSN journal
09717811
Volume
7
Issue
1
Year of publication
1998
Pages
47 - 50
Database
ISI
SICI code
0971-7811(1998)7:1<47:ASMFIP>2.0.ZU;2-3
Abstract
Three methods for in vivo protein labelling of rice anthers were teste d. The 'wick' method was found to incorporate 5- and 9-fold higher amo unt of labelled S-35-methionine into proteins as compared to the other two methods. This method was quite effective, safe and easy to use wi th any kind of tissue or plant species with reproducible results. Two dimensional polyacrylamide gel electrophoresis the qualitative changes in rice anther protein synthesis under water stress was used to test the reproducibility of the method.