C. Pradhan et al., RAPID IN-VITRO PROPAGATION OF EAST-INDIAN ROSEWOOD (DALBERGIA-LATIFOLIA ROXB.) THROUGH HIGH-FREQUENCY SHOOT PROLIFERATION FROM COTYLEDONARYNODES, Journal of Plant Biochemistry and Biotechnology, 7(1), 1998, pp. 61-64
An efficient protocol is described for large scale in vitro propagatio
n of east Indian rosewood (Dalbergia latifolia Roxb.) using cotyledona
ry nodes derived from axenic seedlings. Of the four different cytokini
ns tested, BA was most effective in inducing multiple shoot buds in th
e explants. High frequency shoot proliferation (93%) coupled with maxi
mum number of shoot formation (10-12 shoots/explant) was recorded on M
urashige and Skoog's medium supplemented with 2.0 mg/l BA. The frequen
cy of shoot proliferation declined at higher levels of cytokinins. Sho
ot culture was multiplied by subculturing the original cotyledonary no
de on a fresh shoot multiplication medium each time after excising the
newly formed shoots. Shoots obtained from each passage were multiplie
d further as nodal explants and each node produced 3-4 shoots. In two
months from a single cotyledonary node, about 90 shoots were obtained.
Rooting was induced in 72% of the regenerated shoots on half-strength
MS containing IAA, IBA and IPA each at 1.0 mg/l. Rooted plantlets wer
e hardened off and eventually established in soil.