INFLUENCE OF CHRONIC NITRIC-OXIDE INHIBITION OF CORONARY BLOOD-FLOW REGULATION - A STUDY OF THE ROLE OF ENDOGENOUS ADENOSINE IN ANESTHETIZED, OPEN-CHEST DOGS
S. Tayama et al., INFLUENCE OF CHRONIC NITRIC-OXIDE INHIBITION OF CORONARY BLOOD-FLOW REGULATION - A STUDY OF THE ROLE OF ENDOGENOUS ADENOSINE IN ANESTHETIZED, OPEN-CHEST DOGS, Japanese Circulation Journal, 62(5), 1998, pp. 371-378
The effect of chronic inhibition of endothelium-derived nitric oxide (
NO) synthesis on the regulation of coronary blood flow (CBF) is yet to
be elucidated. A chronic canine model of inhibited NO synthesis was c
reated and the role of adenosine in the regulation of coronary blood f
low in this model was examined. Dogs were fed a diet supplemented with
40 mg/kg per day N-G-nitro-L-arginine methyl eater (L-NAME group, n=8
) or a regular diet without L-NAME supplementation (control group, n=8
) for 4 weeks. The experiments were performed in an anesthetized, open
-chest state and the results were compared in the L-NAME and control g
roups. Chronic L-NAME treatment significantly increased arterial press
ure. Neither basal CBF in the left anterior descending artery nor hear
t rate differed between the L-NAME and control groups. In the L-NAME g
roup, the response of CBF to intracoronary acetylcholine and adenosine
was blunted, but that to glyceryl trinitrate was not. In addition, my
ocardial reactive hyperemia following 20 sec coronary occlusion was bl
unted in the L-NAME group. During atrial pacing at a rate 60 beats/min
faster than the sinus rate, CBF increased to a similar degree in the
L-NAME and control groups, and systolic wall thickening (SWT) changed
similarly in both groups. Intracoronary 8-phenyltheophylline (8-PT), a
n adenosine receptor blocker, decreased basal CBF in the L-NAME group
but not in the control group. In the L-NAME group, pacing-induced incr
ease in CBF was abolished and SWT deteriorated after 8-PT administrati
on. Basal myocardial adenosine release was significantly increased in
the L-NAME group compared with the control group. It is concluded that
in anesthetized, open-chest dogs with chronic inhibition of NO synthe
sis, adenosine may play a compensatory role in the regulation of coron
ary blood flow, as concomitant blockade of adenosine causes deteriorat
ion of coronary circulation and cardiac function.