PHYSICOCHEMICAL CHARACTERISTICS OF SELF-AGGREGATES OF HYDROPHOBICALLY-MODIFIED CHITOSANS

Chitosan derivatives containing 2.8-5.1 deoxycholic acid groups per 10 0 anhydroglucosamine units of chitosan were synthesized by an EDC-medi ated coupling reaction. Physicochemical properties of self-aggregates of the hydrophobically modified chitosans in aqueous media were studie d by the dynamic light scattering method and fluorescence spectroscopy . The mean diameter (d(H)) of DC4.2 self-aggregates in phosphate-buffe red saline (PBS) solution (pH 7.2) was 159 nm with unimodal size distr ibution (variance = 0.082). It seemed that the interparticle interacti on between self-aggregates was almost negligible. The mean diameter de creased with increasing degree of substitution (DS), pH, or ionic stre ngth of the medium; The critical aggregation concentration (cac) of se lf-aggregates was determined from the fluorescence emission spectra of pyrene. The cac value of DC4.2 in PBS solution (pH 7.2) was 2.6 x 10( -2) mg/mL. The cac values decreased with increasing DS, pH, or ionic s trength of the medium.