HIGH-TEMPERATURE GAS-CHROMATOGRAPHY AND HIGH-TEMPERATURE GAS-CHROMATOGRAPHY - NEGATIVE CHEMICAL-IONIZATION MASS-SPECTROMETRY OF DERIVATIZEDTRIGLYCERIDES CONTAINING OXYGENATED FATTY-ACID ACYL-GROUPS

Five seed oils consisting of unusual triacylglycerols have been examin ed by high temperature gas chromatography using glass capillary column s coated with the stationary phase SOP-50 (50%-diphenyl-50%-dimethylpo lysiloxane, methoxy-terminated). The investigated hydroxy, epoxy, and ketokonjuene triacylglycerols were first derivatized in order to permi t analysis by high temperature gas chromatography. Structural elucidat ion of the intact triacylglycerols was performed with high temperature gas chromatagraphy/negative chemical ionization-mass spectrometry usi ng NH3 as a reagent gas. Individual derivatized lipid species with a m olecular weight up to 1176 g/mol could be identified.