R. Kuldvee et al., IMPROVEMENT OF SIGNAL-TO-NOISE RATIO OF ELECTROPHEROGRAMS AND ANALYSIS REPRODUCIBILITY WITH DIGITAL SIGNAL-PROCESSING AND MULTIPLE INJECTIONS, HRC. Journal of high resolution chromatography, 21(3), 1998, pp. 169-174
Poor signal-to-noise ratios and analytical reproducibility in capillar
y electrophoresis were improved by application of multiple injections
of the same sample at (pseudo) randomly chosen time moments. One order
of signal-to-noise ratio improvement was achieved in practice using a
n in-house developed computer-controlled pneumatic sampler. However, i
t is demonstrated here that better implementation of the capabilities
of multiple input requires that the detector signal noise be ''pre-whi
tened'', i.e. spikes, baseline drift, oscillations etc, must be remove
d from the detector signal before transforming it to the electropherog
ram in the common form. Such filtering of the detector signal was real
ized by implementing iterative, menu-driven software written in Matlab
, a high-level programming language.