GENOME SIZES OF THE ENTOMOPATHOGENIC NEMATODES STEINERNEMA-CARPOCAPSAE AND HETERORHABDITIS-BACTERIOPHORA (NEMATODA, RHABDITIDA)

Citation
E. Grenier et al., GENOME SIZES OF THE ENTOMOPATHOGENIC NEMATODES STEINERNEMA-CARPOCAPSAE AND HETERORHABDITIS-BACTERIOPHORA (NEMATODA, RHABDITIDA), Parasitology, 114, 1997, pp. 497-501
Citations number
22
Categorie Soggetti
Parasitiology
Journal title
ISSN journal
00311820
Volume
114
Year of publication
1997
Part
5
Pages
497 - 501
Database
ISI
SICI code
0031-1820(1997)114:<497:GSOTEN>2.0.ZU;2-4
Abstract
There is currently no information on the genome size and complexity in the entomoparasitic nematodes Steinernema and Heterorhabditis. DNA re association kinetics were used to determine the genome size and comple xity in 2 species: Steinernema carpocapsae and Heterorhabditis bacteri ophora. C(0)t curves derived from renaturation kinetics of denatured D NA indicate that the genomes of these entomoparasitic rhabditids are d ifferent both in size and complexity. Genome sizes were estimated at 2 .3 x 10(8) bp for S. carpocapsae and 3.9 x 10(7) bp for H. bacteriopho ra and repetitive DNA contents were found to represent 39% and 51% of these respective genomes.