BIOCHEMICAL CLASSIFICATION OF KIDNEY CARCINOMA BIOPSY SAMPLES USING MAGIC-ANGLE-SPINNING H-1 NUCLEAR-MAGNETIC-RESONANCE SPECTROSCOPY

High resolution H-1 nuclear magnetic resonance (NMR) spectra using spi nning at the magic angle (H-1 MAS NMR) have been obtained on intact no rmal and pathological kidney tissue samples from patients undergoing s urgery for renal cell carcinoma (RCC). The spectra were measured on ca . 80 mg samples and provided high resolution H-1 NMR spectra in which effects of dipolar couplings, chemical shift anisotropy aid magnetic s usceptibility differences are minimised thus yielding high spectral re solution. Conventional one-dimensional and spin-echo spectra and two-d imensional J-resolved, TOCSY and H-1-C-13 HMQC spectra were also measu red on selected samples and these allowed the assignment of resonances of endogenous substances comprising both cytosolic and membrane compo nents. The tumour tissues were characterised principally by all increa sed lipid content. These are the first reported results on human tumou r tissues using this technique and the approach offers potential for t he rapid classification of different types of tumour tissue. (C) 1998 Elsevier Science B.V. All rights reserved.