DETECTION AND IDENTIFICATION OF BETA-1,3- 1,6-GLUCOOLIGOSACCHARIDES IN LAMINARIOLIGOSACCHARIDE MIXTURES BY MEANS OF ENZYMATIC TRANSGLYCOSYLATION - OLIGOSACCHARIDES WITH POLYMERIZATION DEGREE OF 5-6

1 --> 3;1 --> 6-beta-D-Glucooligosaccharides with polymerization degre e of 5-6 were obtained from laminaran and analyzed by the previously d eveloped method [1] by using them as donors and p-nitrophenyl beta-D-g lucoside as the acceptor in the transglycosylation reaction catalysed by endo-1 --> 3-beta-D-glucanase LIV. The resulting homologous p-nitro phenyl beta-1,3-laminarioligosides with polymerization degree of 2-6 a nd the corresponding derivatives of mixed beta-1,3;1,6-glucooligosacch arides with the same polymerization degree were analyzed by HPLC. The latter compounds exhibited higher retention times than the former with the same polymerization degree. Isomeric tetra-, penta-, and hexameri c compounds were detected, and some of them were structurally characte rized by means of NMR. The suggested method of analysis of oligosaccha ride mixtures is simple, informative, and consumes a minimal quantity of sample.