SIMULTANEOUS DETERMINATION OF CYTOTOXIC (ADRIAMYCIN, VINCRISTINE) ANDMODULATOR OF RESISTANCE (VERAPAMIL, S-9788) DRUGS IN HUMAN-CELLS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AND ULTRAVIOLET DETECTION

Authors
TASSIN JP DUBOIS J ATASSI G HANOCQ M
Citation
Jp. Tassin et al., SIMULTANEOUS DETERMINATION OF CYTOTOXIC (ADRIAMYCIN, VINCRISTINE) ANDMODULATOR OF RESISTANCE (VERAPAMIL, S-9788) DRUGS IN HUMAN-CELLS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AND ULTRAVIOLET DETECTION, Journal of chromatography B. Biomedical sciences and applications, 691(2), 1997, pp. 449-456
Citations number
16
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Journal of chromatography B. Biomedical sciences and applications → ACNP
ISSN journal
13872273
Volume
691
Issue
2
Year of publication
1997
Pages
449 - 456
Database
ISI
SICI code
0378-4347(1997)691:2<449:SDOC(V>2.0.ZU;2-4
Abstract
Multidrug resistance (MDR), which was described for structurally and m echanistically unrelated anticancer agents, was modulated in vitro by a series of compounds which were of different chemical origin. In this situation, the selection of a correct assay dosage to study the MDR m odulation mechanism was a problem. We developed a high-performance liq uid chomatography (HPLC) method which enabled the simultaneous determi nation of three major cytotoxins (adriamycin, daunorubicin, vincristin e) and two well-known modulators (S 9788, verapamil). This assay was f ully validated and was used to follow, for the first time, the uptake and accumulation behaviour of adriamycin and S 9788 co-incubated with resistant and sensitive cell lines (KB-3-1; KB-A1).