UP-PCR GENOTYPING AND RDNA ANALYSIS OF ASCOCHYTA-PISI LIB

Isolates of Ascochyta pisi, previously identified as separate pathotyp es, were genotyped by rDNA-RFLP and UP-PCR using 8 UP-primers and 2 ar bitrary primers individually or in pair-wise combinations. The appeara nce of polymorphic UP-PCR products for all studied isolates facilitate d their differentiation. The markers were suitable in identifying isol ates, and may be useful for developing isolate-or pathotype-specific P CR-based diagnostic assays. In studying genetic relatedness by UP-PCR among A, pisi isolates, two distinct clusters were revealed. rDNA anal ysis of the isolates based on endonuclease digestion of amplified ITS1 and ITS2-25S nuclear rDNA regions did not separate the isolates, wher eas the variability of the isolates using UP-PCR was more than 40%.