ANTIFREEZE PROTEINS BIND INDEPENDENTLY TO ICE

Citation
Ci. Deluca et al., ANTIFREEZE PROTEINS BIND INDEPENDENTLY TO ICE, Biophysical journal, 74(3), 1998, pp. 1502-1508
Citations number
30
Categorie Soggetti
Biophysics
Journal title
ISSN journal
00063495
Volume
74
Issue
3
Year of publication
1998
Pages
1502 - 1508
Database
ISI
SICI code
0006-3495(1998)74:3<1502:APBITI>2.0.ZU;2-X
Abstract
It has been suggested that cooperative interactions between antifreeze proteins (AFPs) on the ice surfaces are required for complete inhibit ion of ice crystal growth. To test this hypothesis, a 7-kDa type III A FP was linked through its N-terminus to thioredoxin (12 kDa) or maltos e-binding protein (42 kDa). The resultant 20-kDa and 50-kDa fusion pro teins were larger in diameter than free AFP and thus precluded any ext ensive AFP-AFP contacts on the ice surface. Both fusion proteins were at least as active as free AFP at virtually all concentrations tested. By these criteria, AFPs function independently of each other and do n ot require specific intermolecular interactions to bind tightly to ice .