Zy. Tao et al., COMPARATIVE-STUDY OF ADSORPTION-ISOTHERMS OF BSA ON DEAE-DEXTRAN-TYPEAND CM-DEXTRAN-TYPE ION-EXCHANGERS, Reactive & functional polymers, 32(3), 1997, pp. 257-262
Equilibrium isotherms for adsorption of bovine serum albumin (BSA) on
the weak base (DEAD) dextran-type and the weak acid (CM) dextran-type
ion exchangers were determined experimentally. The constant pH value a
nd ionic strength of BSA solutions respectively remained with differen
t buffers and NaCl, and it was found that the isotherms were affected
by pH considerably. Under certain conditions, the kink or step in the
adsorption isotherms, i.e. the stepwise nature for BSA adsorption was
observed. From the comparison between the adsorption properties of BSA
on DEAE- and CM-ion-exchange resins, the adsorption mechanism of BSA
on dextran-type ion exchangers was discussed.